However this is typically a result of unspecific hybridisation. Due to high amount of the protein in each ladder band, the ladder might show positive results in immunodetection of blots.Use the ladder for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly. Includes 11 protein bands from 8 to 260 kDa (see figure). Use a bit less of the ladder (2-3 µl) for Western blots The Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection.Take 3-5 µl of the ladder for a small size gel slot for electrophoresis.Room-temperature-stable ladders, always ready to use The ScanLater Western Blot Protein Ladder Reagent is designed to be used with the following instruments: SpectraMax® Paradigm® Multi-Mode Detection Platform.Monitoring of electrophoresis process and Western transfer efficiency.Approximate protein molecular weight determination on denaturing gels.

It contains: 62.5 mM Tris-HCl, pH 6.8, 2 (w/v) SDS, 250 mM NaCl. – Research Fellow, Imperial College London protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Protein ladders, also known as protein markers or protein standards, are used to estimate the size of proteins separated in electrophoresis. We could use as little as 5µl which makes these markers cost effective. The ladders also transfer well to membranes for use in Western Blotting and show up clearly in our Biorad imager. The Blue Ultra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying Western transfer. The traditional method to visualize protein ladders on Western blots is to use prestained protein ladders which remain visible when transferred to the blotted membrane. I have struggled to find markers which go over 250kDa of high quality and the CozyXL is by far the best one I have used so far.

The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add. The ladders give nice distinct bands and stain very well using silver stain, not at all blurry and overstained which some of the ladders from market leaders do. The Xpert 2 Prestained Protein Marker consists of a marker with 12 clearly identifiable bands at convenient molecular weights. Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. We have recently bought and tested the CozyHi and CozyXL protein ladders for SDS-PAGE.

Protein ladder western blot